By easily using BLAST to check the translated amino acid sequence from within Snapgene, we can confirm the identity of the gene and check for any mutations.
Snapgene also provides easy visualization of open reading frames, which is often our first clue about where the gene of interest might be located. Next, we use the “Detect Common Features” function to find and annotate promoters, selectable markers, tags, origins of replication, and fluorescent proteins that are already included in Snapgene’s extensive database. The first thing that the Snapgene software will confirm for us is whether it is a linear or circular sequence. We then copy the contents of the resulting FASTA file into the desktop version of Snapgene to visualize the sequence and look for features. While our quality control process for incoming plasmids can vary based on the contents of a plasmid, the first step is always to send prepped plasmid DNA for next generation sequencing.
#Snapgene viewer ipad verification#
To design experiments for further plasmid verification.To align NGS sequencing results with reference sequences.The main ways we use SnapGene for plasmid quality control are: In this blog, we’ll walk you through how a scientist at Addgene uses Snapgene to confirm the sequence of a plasmid and we’ll highlight some of the new features available on our website through our Snapgene powered maps and sequence analysis tools. We regularly use Snapgene for our quality control process because of its expansive feature library and useful tools. We’ve also talked about how our new Snapgene generated maps provide improved feature detection with an easy to use interface. We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell and going into detail about how we use next generation sequencing results to perform quality control on deposited plasmids.
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